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The two protein bands from forty kDa to thirty kDa were absent soon after 24 h incubation at 45 °C. For the rest of the bands, many concentrations of all three proteases retained protein bands akin to their primary molar masses (Figure 6a,b). The presence of bands at very low molecular excess weight in fish roe ahead of hydrolysis was unanticipated. Within the report by Ghelichi et al. [8], minimal peaks of a comparatively minimal molecular body weight range (3–14 kDa) ended up noticed in defatted carp roe homogenate, potentially as a result of an autolytic approach developing in fish roe and/or the defatting procedure resulting in the formation of very low-molecular-pounds peptides.
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